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Decades ago, Otto Warburg discovered a dramatic increase in glucose uptake and lactate production by cancerous cells. ![]() The metabolism in cancer is reprogrammed to accommodate the high energy demands for the highly proliferating cells required for biomass buildup and tumor growth. It highlights a strong association between the ability of asparagus, green tea, rue, and avocado to sensitize HepG2 cells to doxorubicin and their power to amend cell metabolism, suggesting their use as add-on agents that might aid in clinically lowering the doxorubicin dose. In conclusion, our study confirms the potential cytotoxic effects of these plant extracts. Undesirably, green tea extract increased glutaminase avocado oil rose c-Myc, and both increased G6PD. ![]() To less extent, HIF1α, c-Myc, PKM2, and LDH-A were partially inhibited by green tea extract, and HIF1α and glutaminase activity was inhibited by avocado oil. Biochemical investigation displayed several favorable effects, including the inhibition of hypoxia-inducible factor1α (HIF1α), c-Myc, pyruvate kinase-M2 (PKM2), lactate dehydrogenase-A (LDH-A), glucose-6-phosphate dehydrogenase (G6PD), and glutaminase by asparagus and rue extracts. The investigated extract’s ultra-performance liquid chromatography and gas chromatography coupled with mass spectrometry (UPLC/MS and GC/MS) revealed several constituents with anticancer activity. MTT assays elucidated a significant decrease in doxorubicin-IC 50 following HepG2 incubation with each extract, albeit to a variable extent. Here, we incubated HepG2 cells with four plant extracts that were selected based on an earlier assessment of their cytotoxicity, viz asparagus, green tea, rue, and avocado, separately, before treatment with doxorubicin. 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But these days there’s a new kid on the block, the one and only Duolingo, that comes with a smart interface, is simpler, and has two main reasons to be the number one test for applicants, is easier and cheaper! ![]() So once you know that you need to take the English proficiency test, the options used to be TOEFL and IELTS, both tests are the cornerstone to evaluating your English level. When students want to study abroad either for a high school, a university, a master or any degree that they are looking for they usually have to take an English language proficiency test as part of the admissions requirement.įor people that want to live abroad and are looking for a residency in countries such as the United States, Canada, or the United Kingdom they will also need to pass the English proficiency test, depending on the country the score will vary. ![]() ![]() These printers are built to last, with a replaceable printhead and a 2-year onsite warranty, giving you peace of mind knowing that you can secure help if and when needed. With remote printing capability and the HP Smart App, you can print from anywhere, anytime, hassle-free And with a page yield of up to 8,000 color or 6,000 black pages of Original HP Ink included in the box per purchase, you’ll surely get your money’s worth. 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